Limnipivirus A

The
Picornavirus
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Genera

Bluegill picornavirus 1 (BGPV-1) has been isolated from the bluegill fish (Lepomis macrochirus). The bluegill is also known as bream, blue bream, sun perch, blue sunfish, copperhead, copperbelly or roach and lives in streams, rivers and lakes throughout North America.

BGPV-1 particles are icosahedral and average 30 nm in diameter. The virus can be grown in a cell line, BF-2, derived from bluegill fry. The genome layout is typical of a picornavirus except that there appears to be at least two 2A polypeptides each having an NPG↓P motif. The first is predicted to be 17 aa analogous to the FMDV 2A, while the second is 136 aa in length. BGPV-1 may represent a novel picornavirus genus. Within the 3’ UTR, there is a poly(C) tract of 21 residues preceding the poly(A) tail. The complete genome sequence is now available: JX134222 (Barbknecht et al., 2014).

Genome organisation

VPg+5'UTR^IRES-IV[1AB-1C-1D-2A^npgp/2A2^npgp/2B-2C/3A-3B-3C^pro-3D^pol]3'UTR-poly(A)

References

Barbknecht, M. (2009). Characterization of a unclassified virus and survey for its presence in Wisconsin bluegill populations. MSc Thesis, University of Wisconsin-La Crosse, 89 pp.

Barbknecht, M., Sepsenwol, S., Leis, E., Tuttle-Lau, M., Gaikowski, M., Knowles, N.J., Lasee, B. and Hoffman, M.A. (2014). Characterization of a new picornavirus isolated from the freshwater fish Lepomis macrochirus. J. Gen. Virol. 95: 601-613 [Epub 2013 Dec 11].

Berthiaume, L., Robin, J. and Alain, R. (1982). Electron microscopic study of bluegill virus. Can. J. Microbiol. 28: 398-402.

Leis E, Erickson S, McCann R, Standish I, Katona R, Brecka B, Baumgartner W. Bluegill Picornavirus isolated from a mortality event involving Bluegill (Lepomis macrochirus) in the upper Mississippi River. J Fish Dis. 2019 Jun 18. doi: 10.1111/jfd.13046. [Epub ahead of print] PubMed PMID: 31210360.

Mor, S.K., Phelps, N.B., Barbknecht, M., Hoffman, M.A. and Goyal, S.M. (2015). A multiplex RT-PCR assay for the detection of fish picornaviruses. J. Virol. Methods 221: 131-134.

Robin, J. and Dery, C. (1982). The genome of Bluegill virus. Can. J. Microbiol. 28: 58-64.

Robin, J. and Lariviere-Durand, C. (1983). Bluegill virus is a ribovirus of positive-strand polarity. Arch Virol. 77: 119-125.

Robin, J., Lariviere-Durand, C., Berthiaume, L. (1982). Plaque assay of bluegill virus using a methylcellulose overlay. J. Virol. Methods. 5: 351-354.

Wolf, K. (1988). Fish Viruses and Viral Diseases. Cornwell University Press, Ithaca, 476 pp.