Virus name: Grouper virus
Greasy grouper, Epinephelus tauvina
Hegde, A., Chen, C.L., Qin, Q.W., Lam, T.J. and Sin, Y.M. (2002).
Characterization, pathogenicity and neutralization studies of a nervous necrosis
virus isolated from grouper, Epinephelus tauvina, in Singapore.
Aquaculture 213: 55-72.
A virus isolated from diseased marine fish, Grouper, Epinephelus
tauvina, was cultured in sea bass (SB) cell line, characterized and its
pathogenicity and neutralization studies were carried out. This isolated virus
is an icosahedral virus with a mean diameter of 28-30 nm and has buoyant density
of 1.30-1.35 g/ml. It replicates exclusively in the cytoplasm and forms
paracrystalline array and inclusion bodies in the infected cells. SDS-PAGE
analysis of purified virus structural proteins resolved one major polypeptide of
approximately 42 kDa. This virus induces cytopathic effects such as rounding and
granulation of cells, localized cell death and detachment of cells within 3-5
days postinfection on sea bass larval cell line. Typical histopathological
changes in the virus-infected sea bass larvae under experimental conditions
showed vacuolation of nervous tissue. Polyclonal antisera raised against the
purified virus and the coat protein of this virus effectively neutralized virus
infectivity in vitro suggesting the use of coat protein as a vaccine against
this viral infection.
Virus name: unnamed
grouper, Epinephelus malabaricus
Boonyaratpalin, S., Supamattaya, K., Kasornchandra, J. and
Hoffmann, R.W. (1996). Picorna-like
virus associated with mortality and a spongious encephalopathy
in grouper Epinephelus malabaricus. Dis. Aquatic Org.
A picorna-like virus was detected from diseased fry and
fingerling grouper Epinephelus malabaricus
cultured in southern Thailand. Affected grouper
exhibited dark coloration of the head and tail, loss of
equilibrium and corkscrew-like swimming. Pathological
changes of affected fish showed vacuolating
encephalopathy and retinopathy. Intracytoplasmic
inclusions were observed in the nerve cells. From
electron microscopic studies, virus particles could be
detected in the brain and eyeball of affected fishes.
They were round to icosahedral, without an envelope, 20
to 25 nm in diameter and had an electron dense core of
16 nm. Filtrates (0.45 pm) of affected brain and eyeball
tissues can produce the same clinical signs in healthy
grouper after injection, and the identical virus
particles could be observed in the brain and retina of
experimentally infected fish.